A miniprep procedure for isolating genomic DNA from Magnoporthe grisea
نویسندگان
چکیده
منابع مشابه
A simplified genomic subtractive procedure for isolating deleted sequences.
New approaches have recently been developed for directly identifying genes without knowledge about their function and/or position in a genome (1–3). Among these, representational difference analysis (RDA) (2) is widely used in the detection of deleted DNA in cultured cancer cells (4). However, the procedure is complicated and time-consuming. First, preparation of the tester and the driver invol...
متن کاملA rapid procedure for extracting genomic DNA from leukocytes.
1. Collect 5 ml of blood in a vacutainer tube (Becton Dickinson) containing EDTA and mix. 2. Make volume up to 10 ml with solution 1 (10 mM Tris pH 7.6; 10 mM KC1; 10 mM MgCy. 3. Add 120 /il Nonidet P40 (BDH) to lyse the cells. Mix well by inverting several times. 4. Spin down the nuclear pellet at 2000 rpm for 10 mins. 5. Pour off the supernatant without dislodging the pellet. The pellets can ...
متن کاملA simplified high yielding miniprep genomic DNA extraction protocol for three chemotypically different plant species
The presence of metabolites has been observed to create interference with DNA isolation procedures and downstream reactions, such as, DNA amplification, restriction and cloning. Moreover, the chemotypic heterogeneity among species may cause hindrance in optimal DNA yields with a single protocol. Thus, even closely related species may require different isolation protocols. A rapid and high yield...
متن کاملNote on New Procedure for Isolating Ferritin from Horse Liver
In the previous paper (1) a procedure was outlined for the isolation of a highly purified, apparently crystalline, blue-green colored copper protein from horse liver. The precipitate obtained in step 8 of the isolation procedure (1), contains a number of proteins of potential biochemical interest. Two of these proteins, in addition to the copper protein, have been separated by fractionation wit...
متن کاملImproved Protocol for Isolation of Genomic DNA from Leaf Tissues of Phyllanthus emblica Gaertn
Modified Cetyltrimethylammonium bromide (CTAB) protocol for DNA isolation was developed from leaf tissuesof Phyllanthus emblica for obtaining high quality genomic DNA. Fresh leaves of three different maturitywere analyzed for yield and quality of DNA. Acidity was determined in three different maturity of leaves viz.tender, intermediate and mature and their influence on DNA qua...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Fungal Genetics Reports
سال: 1990
ISSN: 1941-4765
DOI: 10.4148/1941-4765.1493